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KMID : 0379520100260010015
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한국독성학회지
2010 Volume.26 No. 1 p.15 ~ p.19
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Identification of Differentially Expressed Genes in Human Mesenchymal Stem Cell-Derived Neurons
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Heo Ji-Hye
Jo Kyung-Jin
Choi Dal-Woong
Kim Suhng-Wook
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Abstract
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Mesenchymal stem cells (MSCs) have greater potential for immediate clinical and toxicological applications, due to their ability to self-renew, proliferate, and differentiate into a variety of cell types. To identify novel candidate genes that were specifically expressed during transdifferentiation of human MSCs to neuronal cells, we performed a differential expression analysis with random priming approach using annealing control primer-based differential display reverse transcription-polymerase chain reaction approach. We identified genes for acyl-CoA thioesterase, tissue inhibitor of metalloproteinases-1, brain glycogen phosphorylase, ubiquitin C-terminal hydrolase and aldehyde reductase were up-regualted, whereas genes for transgelin and heparan sulfate proteoglycan were down-regulated in MSC-derived neurons. These differentially expressed genes may have potential role in regulation of neurogenesis. This study could be applied to environmental toxicology in the field of testing the toxicity of a chemical or a physical agent.
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KEYWORD
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Mesenchymal stem cells, Neuron, Differentially expressed genes
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